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During the Anti-Japanese War, cholera bacteria were one of the most widely used bacterial weapons by the Japanese bacterial warfare troops. The cholera bacteriological warfare carried out by the Japanese invaders was an important source of the cholera pandemic during China's Anti-Japanese War, and the serious harm caused must be exposed. The first chapter of this book summarizes the history of cholera epidemics in various parts of the world and in China before the Anti-Japanese War; the second chapter examines the research activities of the Japanese invaders on the weaponization of cholera bacteria; the third chapter examines the cholera bacterial warfare experiments and combat methods and technologies of the Japanese invaders from 1937 to 1941. Formed; Chapters 4 to 6 examine the Japanese military's cholera bacteriological warfare in China year by year from 1942 to 1945; Chapter 7 generally discusses the characteristics, patterns, consequences and hazards of the Japanese cholera bacteriological warfare, providing a reference for continued in-depth study of the history of Japanese cholera bacteriological warfare. This book believes that from 1938 to 1945, the Japanese invaders carried out more than 20 cholera bacteriological warfare activities in China, covering 20 provinces and cities, causing hundreds of thousands of Chinese people to suffer harm, including at least 160,000 people who died from the Japanese cholera bacteriological warfare. This book is a systematic investigation of the cholera bacteriological warfare of the Japanese invaders in China. It has important academic value for studying and discussing the relationship between the cholera epidemic in China and the Japanese bacteriological warfare.