- Male protagonist: Gu Yan - 33 years old, deputy chief physician of the Department of Oncology of the First Municipal Hospital. Coming from a medical family, he has a reserved and restrained personality, and is used to wrapping up his emotions with medical records and data. Five years ago, he failed to save his mother who suffered from pancreatic cancer. Since then, he has adopted "not investing too much emotion" as his medical practice. There is a medical notebook left by his mother on his desk all year round. The title page reads "To heal the disease, it is more important to heal the heart." There is a shallow scar on the tiger's mouth of his left hand. It was accidentally scratched when he performed an emergency puncture for his mother. He always subconsciously covers it with a pen.
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- Heroine: Xu Qinghuan - 25 years old, freelance illustrator. He is good at using delicate brushstrokes to outline the glimmers of life, and his works have been selected for international illustration exhibitions. Before she was diagnosed with late-stage gastric cancer (stage IV) with abdominal metastasis, she had just taken over the creation of a children's picture book, and there was still an unfinished fairy tale town on the canvas. She carried a worn canvas bag with her, which contained sketchbooks, different types of paintbrushes, stomach medicine, and a yellowed photo - it was a photo of her and her long-dead grandmother in an old alley, holding the first painting she drew when she was a child.
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- Lin Wei - 32 years old, Gu Yan's partner nurse. She has a straightforward and enthusiastic personality. She has witnessed the whole process of the two of them from doctors and patients to confidants. She often secretly helps Su Nian bring her favorite caramel macchiato (without the ice and sugar).
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- Chen Yu - 29 years old, Su Nian's childhood, runs an independent bookstore, and goes to the hospital every week with new books and the sweet-scented osmanthus cake that Su Nian loves to eat. It is her "link" to the outside world, and also knows her hidden wish - to hold an illustration exhibition of her own.
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- Professor Zhang - 60 years old, dean of medical oncology and Gu Yan's mentor. He noticed Gu Yan's special feelings for Su Nian and often reminded him that "a doctor's rationality and emotion are never contradictory."
Mission: Medicine is not only about saving lives, but also about caring for patients' spiritual world.